脂肪細胞分化初期に発現が変動する遺伝子群の単離及び機能解析

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  • 西塚　 誠
    <i>名古屋市立大学大学院薬学研究科分子生物薬学分野</i>

書誌事項

タイトル別名
  • Cloning and Characterization of Inducible Genes at the Beginning of Adipocyte Differentiation
  • シボウ サイボウ ブンカ ショキ ニ ハツゲン ガ ヘンドウ スル イデンシグン ノ タンリ オヨビ キノウ カイセキ

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抄録

  Adipocyte differentiation takes place via a complex series of steps. While PPARγ and C/EBPα are known to be master regulators, the events at the earliest stage of adipocyte differentiation are not yet known. In this study, we cloned the genes that are induced at the beginning of the differentiation of 3T3-L1 preadipocyte cells. Of 102 clones obtained, only several clones were already reported as genes that are expressed differentially during adipocyte development. The expression of TCL/TC10βL (TC10-like/TC10βLong) and RGS2 (regulators of G protein signaling 2) genes isolated here rapidly increased after the addition of inducers (insulin, dexamethasone, 3-isobutyl-1-methylxanthine, fetal bovine serum[FBS]). Further, the antisense TCL/TC10βL inhibited the adipogenesis of mouse 3T3-L1 preadipocyte cells, prevented cytoplasmic triglyceride accumulation, and decreased the expression of PPARγ and C/EBPα. Moreover, the constitutive overexpression of TCL/TC10βL or RGS2 in the mouse fibroblast cell line NIH-3T3 results in efficient adipocyte conversion when stimulated with 10% FBS, insulin, 3-isobutyl-1-methylxanthine, dexamethasone, and PPARγ ligand BRL49653. These results strongly suggest that TCL/TC10βL and RGS2 have crucial roles in the program of adipocyte differentiation, probably linked to the PPARγ pathway. Using a subtraction protocol, the genes specifically regulated by TCL/TC10βL were also isolated. The expression pattern of some was similar to TCL/TC10βL expression in adipogenesis, suggesting that these genes are regulated by TCL/TC10βL.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 123 (11), 949-956, 2003-11-01

    公益社団法人 日本薬学会

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