Bibliographic Information
- Other Title
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- 市販リン脂質中のグリセロリン酸の分析
- シハン リン シシツチュウ ノ グリセロリンサン ノ ブンセキ
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Abstract
A sensitive and selective gas chromatographic method for the determination of glycerophosphate was carried out and good results were obtained, in which a flame photometric detector (FPD) was used as a detector and the emission was measured with a photomultiplier tube through an interference filter with a transmission maximum at 526 nm. Glycerophosphate was trimethylsilylated with bis (trimethylsilyl) fluoroacetamide containing 1% trimethychlorosilane in pyridine. Tetrakis (trimethylsilyl)-glycerophosphate was subjected to a gas chromatograph equipped with an FPD and a glass column (3 mm i.d.×1 m) packed with 2% OV-17 on Chromosorb W, AW-DMCS. The column temperature was 160°C, the carrier gas was nitrogen at a flow-rate of 40 ml/min, the fuel gas was hydrogen (100 ml/min) and oxygen (20 ml/min), and detector gas was nitrogen (40 ml/min). The photomultiplier voltage was 700 V. Response to glycerophosphate was found to be linear from 1 ng to 1 μg. The retention times of glycerophosphates of GC-FPD were as follows : β-glycerophosphate (3.2 min), α-glycerophosphate (3.8 min). This method was found to be much more rapid and simple than a paper chromatography and a thin-layer chromatography. Thus the method is convenient for the measurement of glycerophosphates in commercial phospholipids.
Journal
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- YAKUGAKU ZASSHI
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YAKUGAKU ZASSHI 101 (8), 749-752, 1981
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001206128996992
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- NII Article ID
- 110003650211
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- NII Book ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL BIB ID
- 2358538
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- Data Source
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- JaLC
- NDL
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- CiNii Articles
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- Abstract License Flag
- Disallowed