書誌事項
- タイトル別名
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- The Mechanisms of the Release of Cytochrome c from Mitochondria Revealed by Proteomics Analysis
- プロテオミクス デ トキアカス ミトコンドリア カラ ノ シトクロム c ホウシュツ キコウ
この論文をさがす
説明
Mitochondrial permeability transition (PT) is the phenomenon in which the mitochondrial inner membrane becomes permeable to various solutes and ions. When PT is induced by Ca2+, cytochrome c is released from mitochondria into the cytosol where it then triggers subsequent steps of programmed cell death, apoptosis. Thus, the proteins that regulate PT and cytochrome c release could become druggable targets for various diseases. However, the mechanisms of PT and the release of cytochrome c have not yet been revealed. We previously showed that valinomycin, a potassium selective ionophore, also caused release of cytochrome c from mitochondria without inducing PT. This result indicates that cytochrome c could be released from mitochondria with or without induction of PT. In this study, to understand the difference of effects of valinomycin and Ca2+ on mitochondria, we examined what protein species are released from valinomycin- and Ca2+-treated mitochondria by LC-MS/MS. As a result, only the proteins located in the intermembrane space were found to be released from valinomycin-treated mitochondria, while those in both the intermembrane space and in the matrix were released from Ca2+-treated mitochondria. Furthermore, the protein releases by each reagent occurred not selectively but in a concentration-dependent manner. Based on these results, the permeabilization effects of Ca2+ and valinomycin on the inner and outer mitochondrial membranes are discussed.<br>
収録刊行物
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- 薬学雑誌
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薬学雑誌 132 (10), 1099-1104, 2012-10-01
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001206129352064
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- NII論文ID
- 130001871979
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- NII書誌ID
- AN00284903
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- COI
- 1:STN:280:DC%2BC3s%2Fis1Cntw%3D%3D
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- ISSN
- 13475231
- 00316903
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- NDL書誌ID
- 024020395
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- PubMed
- 23037694
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可