人工RNAテクノロジーによる哺乳類細胞機能制御

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  • 齊藤 博英
    京都大学iPS細胞研究所初期化機構研究部門 京都大学白眉センター

書誌事項

タイトル別名
  • Synthetic RNA Technologies to Control Functions of Mammalian Cells
  • Symposium Review 人工RNAテクノロジーによる哺乳類細胞機能制御
  • Symposium Review ジンコウ RNA テクノロジー ニ ヨル ホニュウルイ サイボウ キノウ セイギョ

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  We recently succeeded in producing nanostructures made of RNA-protein (RNP) complexes. We show that RNA and the ribosomal protein L7Ae can form a triangular-like nanostructure that consists of three L7Ae proteins, which form the apices of the triangle, bound to one RNA scaffold. This shape is created through a 60° kink introduced into the RNA structure on L7Ae binding. By varying the size of the RNA scaffold we could in turn alter the overall size of the triangular nanostructure. Several functions can be added to this nanostructure by the introduction of effector proteins fused to L7Ae. The design and construction of functional RNP nanostructures that detect specific cancer cells are discussed herein. In parallel, we developed synthetic RNP translational switches to control production levels of particular proteins depending on certain input(s) within the intracellular environment. The RNP-binding module was successfully incorporated into mRNA to generate functional RNP switches. The designed ON/OFF translational switches detect expression of the trigger factor and repress or activate expression of a desired protein (e.g., apoptosis regulator) in target mammalian cells. Taken together, RNP-binding module could be employed for constructing designer genetic switches and functional nanostructures to regulate cellular processes.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 135 (3), 399-404, 2015-03-01

    公益社団法人 日本薬学会

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