アルコール類の蛍光誘導体化試薬7-Dimethylaminocoumarin-3-isocyanateの合成及びその反応性

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タイトル別名
  • Synthesis and Reactivity of 7-Dimethylaminocoumarin-3-isocyanate as a Fluorescent Derivatization Reagent for Alcohols
  • アルコールルイ ノ ケイコウ ユウドウタイカ シヤク 7 Dimethylam

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This paper describes the preparation of 7-dimethylaminocoumarin-3-isocyanate (MACI) having 7-dimethylaminocoumarin as a high sensitive fluorophore together with an isocyanate group as a reacting moiety and a preliminary investigation as a precolumn flourescence derivatization reagent for alcohols in high performance liquid chromatography (HPLC). Alcohols were derivatized quantitatively into the corresponding fluorescent urethanes by treating with MACI at 80°C for 10 min. The derivatives were subjected to HPLC on an Inertsil ODS (250×4.6 mm i.d., 5 μm) column with acetonitrile-ethanol-water (50 : 49 : 1) as mobile phase and monitored with an excitation wavelength of 382 nm and an emission wavelength of 479 nm. The detection limit of cholestanol was 10 fmol for an injection volume of 10 μl.

収録刊行物

  • 薬学雑誌

    薬学雑誌 110 (2), 155-158, 1990

    公益社団法人 日本薬学会

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