A Simultaneous Determination of Evodiamine and Rutaecarpine in Oriental Pharmaceutical Decoctions Containing Evodia Fruit by Ion-Pair High-Performance Liquid Chromatography

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Other Title
  • イオンペアークロマトグラフ法による呉茱萸配合漢方湯液中のEvodiamine及びRutaecarpineの同時定量
  • イオン ペアー クロマトグラフホウ ニヨル ゴシュユ ハイゴウ カンポウ トウ

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Description

A simple and precise method was established for the simultaneous determination of evodiamine and rutaecarpine in oriental pharmaceutical decoctions containing Evodia Fruit using high-performance liquid chromatography with sodium dodecyl sulfate (SDS) as an ion-pair reagent. Evodiamine and rutaecarpine were eluted within 60 min without interference from co-existing components using an ODS column and a mixture of water-methanol-acetonitrile-SDS-phosphoric acid (600 : 330 : 70 : 5 : 0.01, v/v/v/w/v, pH 5.0) as a mobile phase.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 116 (1), 59-64, 1996

    The Pharmaceutical Society of Japan

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