Analysis of Natural Medicines by Micellar Electrokinetic Chromatography(MEKC) : Determination of Calycosin in Astragali Radix

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  • ミセル動電クロマトグラフィーによる生薬分析 : オウギ中のカリコシンの分析
  • ミセル ドウデン クロマトグラフィー ニ ヨル ショウヤク ブンセキ オウギ チュウ ノ カリコシン ノ ブンセキ

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Abstract

We previously reported a HPLC identification method for Astragali Radix and its fluid extract using calycosin as a marker substance. However, it took about 40 min for one run using gradient elution. Therefore, we alternatively employed capillary electrophoresis (CE) for the analysis of calycosin in those materials. As a result, calycosin was clearly separated from the other components in Astragali Radix and its fluid extract by micellar electrokinetic chromatography(MEKC)mode with sodium dodecyl sulfate (SDS) within 5 min. Then we determined the content of calycosin in a drinkable preparation. Validation for the developed method was also performed in accordance with the ICH guideline.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 120 (9), 779-785, 2000

    The Pharmaceutical Society of Japan

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