Studies on Human Prostatic Acid Phosphatase. II. Characterization and Its Application in Clinical Diagnosis of Anti-Human Prostatic Acid Phosphatase Serum

DOI Web Site Web Site

Bibliographic Information

Other Title
  • ヒト前立腺酸性ホスファターゼに関する研究(第2報)抗ヒト前立腺酸性ホスファターゼ血清の性状とその臨床的応用
  • ヒト前立腺酸性ホスファターゼに関する研究-2-抗ヒト前立腺酸性ホスファターゼ血清の性状とその臨床的応用
  • ヒト ゼンリツセン サンセイ ホスファターゼ ニカンスルケンキュウ 2 コウ

Search this article

Abstract

Monospecific antiserum to purified human prostatic acid phosphatase was produced in rabbits. Prostatic acid phosphatase treated with neuraminidase retained all enzyme activity before treatment and reacted with the antiserum. The enzyme treated with urea, sodium dodecyl sulfate, 2-mercaptoethanol and guanidine hydrochloride lost all enzyme activity and did not react with the antiserum. We demonstrated that the antibody to the prostatic acid phosphatase can protect the enzyme specifically against heat and pH inactivation. When the prostatic acid phosphatase was coupled with the antibody, enzyme activity was markedly stabilized at pH 4.6-5.8 at 56°. Prostatic acid phosphatase activities in human sera were assayed using the antibody. The activity values were high in sera of patients with prostatic carcinoma and low in sera of patients with prostatic hypertrophy and normal subjects.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 99 (1), 83-89, 1979

    The Pharmaceutical Society of Japan

Keywords

Details 詳細情報について

Report a problem

Back to top