Studies on Crude Drugs originated from Gentianaceous Plants. I. Determination of Gentiopicroside, the Bitter Principle of Gentianae Radix and Gentianae Scabrae Radix

DOI Web Site Web Site 1 Citations

Bibliographic Information

Other Title
  • リンドウ科植物を基源とする生薬の研究(第1報)ゲンチアナ, リュウタン中の苦味成分Gentiopicrosideの定量
  • リンドウ科植物を基源とする生薬の研究-1-ゲンチアナ,リュウタン中の苦味成分Gentiopicrosideの定量
  • リンドウカ ショクブツ オ キゲン ト スル ショウヤク ノ ケンキュウ 1

Search this article

Description

Two new methods for the determination of gentiopicroside in gentian radix and Gentiana scabra radix were developed by the combination of thin-layer chromatography (TLC) and spectrophotometry. In the preparative TLC-ultraviolet (UV) method, gentiopicroside contained in the methanol extract ofsamples is quantitatively separated by preparative TLC (detection by a PAN UV lamp) and the amount is determined from the absorbance at 270 nm by using a calibration graph. In the TLC-densitometer (DM) method, the methanol extract containing gentiopicroside is developed on the TLC plate, and the amount of gentiopicroside (Rf=ca. 0.4) is measured by TLC densitometry using a dual-wavelength TLC scanner. By these two methods, the content of gentiopicroside vras determined as 1-2% in gentian idix (Osaka market product), 1-3% in Gentiana scabra radix (Osaka market product), and 7-10% in the fresh root of Gentiana scabra BUNGE.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 96 (3), 356-361, 1976

    The Pharmaceutical Society of Japan

Citations (1)*help

See more

Details 詳細情報について

Report a problem

Back to top