Application of DNA Markers to Research on Trichoderma in Mushroom Facilities of Japan (1) : RAPD, SSCP marker
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- MIYAZAKI Kazuhiro
- Forestry and Forest Products Research Institute Kyushu Research Center
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- TSUNODA Mitsutoshi
- Forestry and Forest Products Research Institute
Bibliographic Information
- Other Title
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- 日本のきのこ栽培施設におけるトリコデルマ調査のためのDNAマーカーの適用(第一報) : RAPD,SSCPマーカー
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Description
Trichoderma causes, green mold, and inhibits the harvest of mushroom crops. We analyzed a total of 24 isolates of Trichoderma, Gliocladium and Hypocrea from mushroom facilities and on wild mushrooms, for molecular characteristics. We found random amplified polymorphic DNA (RAPD) markers to be useful for detecting clonal reproductions. A common fragment (A10-2000) was found in the T. harzianum aggregate isolates by RAPD analysis using primer A10. The single strand conformation polymorphisms (SSCP) analysis patterns of the internal transcribed spacer 1 (ITS1) region on the nuclear genome were characteristic of each species and aggregate.
Journal
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- Mushroom science and biotechnology
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Mushroom science and biotechnology 11 (2), 65-70, 2003
Japanese Society of Mushroom Science and Biotechnology
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Details 詳細情報について
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- CRID
- 1390001206222618112
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- NII Article ID
- 110008679516
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- NII Book ID
- AA12493760
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- ISSN
- 24330957
- 13453424
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- NDL BIB ID
- 6727100
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed