Stability of JC Virus DNA in Cerebrospinal Fluid Specimens Preserved with Guanidine Lysis Buffer for Quantitative PCR Testing

DOI Web Site PubMed 2 Citations 17 References
  • Nakamichi Kazuo
    Department of Virology 1, National Institute of Infectious Diseases
  • Lim Chang-Kweng
    Department of Virology 1, National Institute of Infectious Diseases
  • Saijo Masayuki
    Department of Virology 1, National Institute of Infectious Diseases

Search this article

Abstract

Quantitative PCR testing for JC virus (JCV) DNA in the cerebrospinal fluid (CSF) is one of the diagnostic standards for progressive multifocal leukoencephalopathy (PML). The present study was conducted to examine its reliability using CSF specimens that had been preserved with guanidine lysis buffers in commercial nucleic acid extraction kits under different conditions. When CSFs were mixed with guanidine buffers, JCV DNA levels were not statistically reduced even after storage for 1 month at room temperature or for 3 months at −80℃, compared with the control samples. In addition, the JCV DNA level was not decreased in a mixture of CSF and guanidine thiocyanate buffer incubated for 3 days at 56℃. These data suggest that CSF specimens mixed with commercial guanidine buffers can be stored without refrigeration, more safely handled, and directly subjected to JCV DNA testing for PML.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 67 (4), 307-310, 2014

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Citations (2)*help

See more

References(17)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top