Prevalence of Diarrheagenic <i>Escherichia coli</i> in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

  • Wang Lili
    School of Life Science and Biotechnology, Dalian University of Technology Graduate School of Human Life Science, Osaka City University
  • Zhang Shaobo
    Graduate School of Human Life Science, Osaka City University
  • Zheng Dongming
    Graduate School of Human Life Science, Osaka City University
  • Fujihara Sami
    National Hospital Organization Osaka Minami Medical Center
  • Wakabayashi Akiyo
    Hyogo Prefecture Tajima Meat Hygiene Inspection Office
  • Okahata Kazuyuki
    Hyogo Prefecture Tajima Meat Hygiene Inspection Office
  • Suzuki Masakazu
    Hyogo Prefecture Tajima Meat Hygiene Inspection Office
  • Saeki Atsunori
    Osaka Municipal Meat Inspection Center
  • Nakamura Hiromi
    Osaka City Institute of Public Health and Environmental Sciences
  • Hara-Kudo Yukiko
    National Institute of Health Sciences
  • Kage-Nakadai Eriko
    Graduate School of Human Life Science, Osaka City University The OCU Advanced Research Institute for Natural Science and Technology, Osaka City University
  • Nishikawa Yoshikazu
    Graduate School of Human Life Science, Osaka City University

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Other Title
  • Prevalence of Diarrheagenic Escherichia coli in Foods and Fecal Specimens Obtained from Cattle, Pigs, Chickens, Asymptomatic Carriers, and Patients in Osaka and Hyogo, Japan

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Abstract

<p>The source and routes of diarrheagenic Escherichia coli (DEC) remain poorly understood. To investigate the involvement of domestic animals in the dissemination of DEC, the prevalence of DEC in foods and fecal specimens from cattle, pigs, chickens, healthy carriers, and patients in Osaka and Hyogo, Japan was investigated using a multiplex real-time Polymerase Chain Reaction assay. The most abundant virulence genes were astA and eae, which had a prevalence 46.8% and 27.4%, respectively. Additionally, stx1 (26.6%) and stx2 (45.9%) were prevalent in cattle feces, while est (8.5%) and elt (7.6%) were prevalent in pig feces. afaB was the second-most prevalent gene in patients and healthy carriers, and it had detection rates of 5.1% and 8.1%, respectively. In contrast, afaB was not detected in animal feces or foods, except for three porcine fecal samples. The aggR gene was more prevalent in humans than in foods or animal feces. Both Shiga toxin-producing E. coli and atypical enteropathogenic E. coli carried by cattle may be sources for diarrheal diseases in humans. Pigs may be a source for human enterotoxigenic E. coli infections, whereas humans are expected to be the reservoir for diffusely adhering E. coli, enteroaggregative E. coli, and enteroinvasive E. coli.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 70 (4), 464-469, 2017

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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