Identification of a Novel Metallo-β-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in <i>Pseudomonas aeruginosa</i> Isolates from Egypt

El-Mahdy Taghrid S.

doi:10.7883/yoken.67.382

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Identification of a Novel Metallo-β-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in Pseudomonas aeruginosa Isolates from Egypt
Identification of a Novel Metallo-^|^beta;-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in Pseudomonas aeruginosa Isolates from Egypt

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Abstract

Two multidrug-resistant Pseudomonas aeruginosa strains harboring bla_VIM were isolated from a hospital in Egypt and were indistinguishable by pulsed-field gel electrophoresis. Sequence analysis revealed a novel VIM β-lactamase, VIM-28. Additional sequencing of integron revealed that the bla_VIM-28 gene cassette had an unusual arrangement of class 1 integron structure, located directly downstream of the integrase gene “intI1” and oriented divergently from it. The new organization of integron also comprised the aacA7 and smr-2 gene cassettes in that order. A complete ISPa21 containing a tnpA gene flanked by two 13-bp inverted repeats was located directly upstream of the 3′-CS conserved region of the integron containing qacEΔ<i>1/sul1</i> genes. The bla_VIM-28-containing class 1 integron was found to have a chromosomal origin in both strains. In conclusion, the presence of a new variant of bla_VIM, bla_VIM-28, on a new organization of class 1 integron having ISPa21 increases the possibility of dissemination of resistance genes within the integron in P. aeruginosa among hospitalized patients in Egypt.

Journal

Japanese Journal of Infectious Diseases

Japanese Journal of Infectious Diseases 67 (5), 382-384, 2014

National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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