Identification of a Novel Metallo-β-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in <i>Pseudomonas aeruginosa</i> Isolates from Egypt

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  • Identification of a Novel Metallo-β-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in Pseudomonas aeruginosa Isolates from Egypt
  • Identification of a Novel Metallo-^|^beta;-Lactamase VIM-28 Located within Unusual Arrangement of Class 1 Integron Structure in Pseudomonas aeruginosa Isolates from Egypt

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Abstract

Two multidrug-resistant Pseudomonas aeruginosa strains harboring blaVIM were isolated from a hospital in Egypt and were indistinguishable by pulsed-field gel electrophoresis. Sequence analysis revealed a novel VIM β-lactamase, VIM-28. Additional sequencing of integron revealed that the blaVIM-28 gene cassette had an unusual arrangement of class 1 integron structure, located directly downstream of the integrase gene “intI1” and oriented divergently from it. The new organization of integron also comprised the aacA7 and smr-2 gene cassettes in that order. A complete ISPa21 containing a tnpA gene flanked by two 13-bp inverted repeats was located directly upstream of the 3′-CS conserved region of the integron containing qacEΔ<i>1/sul1</i> genes. The blaVIM-28-containing class 1 integron was found to have a chromosomal origin in both strains. In conclusion, the presence of a new variant of blaVIM, blaVIM-28, on a new organization of class 1 integron having ISPa21 increases the possibility of dissemination of resistance genes within the integron in P. aeruginosa among hospitalized patients in Egypt.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 67 (5), 382-384, 2014

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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