Virological Studies of smallpox in an Endemic Area-1-Evaluation of Immunofluorescence Staining as a Rapid Diagnostic Procedure in the Field
書誌事項
- タイトル別名
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- VIROLOGICAL STUDIES OF SMALLPOX IN AN ENDEMIC AREA I. EVALUATION OF IMMUNOFLUORESCENCE STAINING AS A RAPID DIAGNOSTIC PROCEDURE IN THE FIELD
- Virological Studies of smallpox in an E
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説明
Seventy-eight patients under the surveillance of joint IndiaW.H.O. Smallpox Eradication activities in Bihar State, India, were visited and virological specimens were collected by the authors in February and March, 1975. Rapid diagnosis by direct immunofluorescence (IF) staining was attempted against smallpox (SPX), varicella-zoster (VZ) and herpes simplex (HS), type 1, viruses in a field laboratory composed of facilities brought from authors' home laboratories. A procedure applying IF staining to scabs was developed by releasing and smearing the antigen-containing epithelial cells and leukocytes. IF results in the field were compared with virus isolation in the home laboratory with specimens sent back through W.H.O. under rigid freezing in liquid nitrogen:<BR>1) All 50 cases clinically diagnosed of smallpox ranging from macular (2 days after rush onset; vesicular smear) to late scabbing (30 days after rush onset; scab) stages, gave positive SPX.IF staining.<BR>2) Three cases out of four of clinical varicella and three out of nine suspected varicella cases were demonstrated to be VZ.IF positive.<BR>3) Eleven cases were demonstrated to be SPX.IF positive from cases of suspected smallpox (2/3), varicella (2/9), undecided suspects of smallpox or varicella (4/5), combined smallpox and varicella (1/1), suspected measles (1/1) and uncharacteristic eruptive cases (1/5) .<BR>4) Variola virus was isolated from 55 out of 61 SPX.IF-positive cases. No variola virus was isolated from specimens of SPX.IF-negative cases.<BR>From above observations, it was concluded that IF staining should be the first choice of smallpox diagnostic procedure which has both versatility and rapidity in the field with high sensitivity to have no possibility of false negative results.
収録刊行物
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- Japanese Journal of Medical Science and Biology
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Japanese Journal of Medical Science and Biology 30 (5), 215-227, 1977
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001206239960832
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- NII論文ID
- 130003778314
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- NII書誌ID
- AA1132885X
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- COI
- 1:STN:280:CSeD1M%2Fgtlc%3D
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- ISSN
- 18842828
- 00215112
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- NDL書誌ID
- 1846916
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- PubMed
- 202770
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可