ANALYSIS OF HOST-ORIGINATED LIPIDS ASSOCIATING WITH “IN VIVO GROWN TUBERCLE BACILLI”

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  • KONDO EIKO
    <I>Department of Tuberculosis, National Institute of Health</I>
  • KANAI KOOMI
    <I>Department of Tuberculosis, National Institute of Health</I>
  • NISHIMURA KENJI
    <I>Department of Cell Chemistry, Institute for Medical Science, University of Tokyo</I>
  • TSUMITA TORU
    <I>Department of Cell Chemistry, Institute for Medical Science, University of Tokyo</I>

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  • ANALYSIS OF HOST-ORIGINATED LIPIDS ASSOCIATING WITH ^|^ldquo;IN VIVO GROWN TUBERCLE BACILLI^|^rdquo;

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Abstract

1) In order to confirm the preceding observation that “in vivo grown tubercle bacilli” harvested from infected mouse lungs were closely associated with host components, lipid analysis was conducted of this material.<BR>2) The 90% acetone extract contained sphingomyelin as the major phospholipid, the neutral lipids including cholesterol in 20%, and free fatty acids such as palmitic, stearic, oleic and linoleic acids.<BR>3) The 90% acetone-insoluble residue was further extracted with chloro-form-methanol (2: 1, v/v) . From this extract, sphingomyelin and lecithin were demonstrated only in a slight amount, insteads cardiolipin was detected as the major phospholipid. Tuberculostearic acid was present in 25% of the constituent fatty acids of the phospholipid fraction. Cholesterol was still detected, but mostly as the ester.<BR>4) These results, together with those in the preceding paper, suggest that the 90% acetone treatment could remove the major portion of host-originated lipids associating with the bacilli grown in vivo and the chloroform-methanol extracted mostly the components proper to the bacilli.<BR>5) Interesting was an additional observation that the ratio of lecithin to sphingomyelin and the ratio of cholesterol to its ester decreased greatly in the bacillus-associated host lipids, probably as a result of the host-parasite interaction.

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