TITRATION OF KAPPA TOXIN OF <I>CLOSTRIDIUM PERFRINGENS</I> AND KAPPA ANTITOXIN BY A MODIFIED AZOCOLL METHOD

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  • KAMEYAMA SHOICHI
    <I>The 2nd Department of Bacteriology, National Institute of Health</I>
  • AKAMA KIYOTO
    <I>The 2nd Department of Bacteriology, National Institute of Health</I>

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  • Titration of kappa toxin of Clostridium perfringens and kappa antitoxin by a modified azocoll method

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Azo-dye was liberated from azocoll not only by proteolytic enzymes but also by albumin (serum or egg), a culture medium (papain digest of meat) or polyvinylpyrrolidone (PVP) . Pretreatment of azocoll with papain and PVP increased the reactivity of azocoll to κ-toxin of Clostridium perfringens. A rapid and reliable assay method of κ-toxin and κ-antitoxin using the pretreated azocoll is described.

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