PRODUCTION AND CHARACTERIZATION OF ANTIBODIES TO OUABAIN

  • TERANO Yoshitake
    <I>Department of Physiology, Osaka City University Medical School</I>
  • TOMII Akiko
    <I>Department of Geriatrics, Osaka University Medical School</I>
  • MASUGI Fuminori
    <I>Department of Geriatrics, Osaka University Medical School</I>

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Description

Two monoclonal antibodies (249F8 and 278A9) to ouabain were produced by somatic cell fusion. They reacted in dose-dependent manners with ouabain and digoxin. These antibodies were supposed to recognize the partial structure important for cardiac compounds to show their pharmacological activity, Na+, K+-ATPase inhibition in a competitive mode against K+. These monoclonal antibodies may be useful for the immunochemical isolation, the structual elucidation, and the quantitative measurement of putative endogeneous digoxin-like factors.

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Details 詳細情報について

  • CRID
    1390001206240985856
  • NII Article ID
    130003953446
  • DOI
    10.7883/yoken1952.44.123
  • COI
    1:CAS:528:DyaK38Xitlent70%3D
  • ISSN
    18842828
    00215112
  • PubMed
    1664470
  • Text Lang
    en
  • Article Type
    journal article
  • Data Source
    • JaLC
    • Crossref
    • PubMed
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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