Monitoring Genetic Diversity of Influenza A(H1N1)pdm09 Virus Circulating during the Post-Pandemic Period in Turkey
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- Guldemir Dilek
- Department of Microbiology Reference Laboratories, Public Health Agency of Turkey
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- Kalaycioglu Atila T.
- Department of Microbiology Reference Laboratories, Public Health Agency of Turkey
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- Altas A. Basak
- Department of Microbiology Reference Laboratories, Public Health Agency of Turkey
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- Korukluoglu Gulay
- Department of Microbiology Reference Laboratories, Public Health Agency of Turkey
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- Durmaz Riza
- Department of Microbiology Reference Laboratories, Public Health Agency of Turkey Department of Clinical Microbiology, Faculty of Medicine, Kirikkale University
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The aimes of the present study were to monitor genetic alterations in the hemagglutin (HA) gene and oseltamivir resistance-related alterations in the neuraminidase (NA) gene of influenza A(H1N1)pdm09 viral isolates detected during the post-pandemic period in Turkey. A total of 2601 clinical specimens obtained from suspected cases of influenza A(H1N1)pdm09 viral infections were analyzed by real-time reverse transcription polymerase chain reaction. Viral RNA was detected in 233 (9%) clinical specimens. Sequence analysis of the HA gene in 16 random isolates showed >98.7% homology among each other and with the A/California/07/2009 vaccine strain. These 16 isolates had common (75%–100%) amino acid substitiutions at positions P83S, D97N, S203T, R205K, I216V, V249L, I321V, and E374K in the HA gene. In addition, two additional rare mutations were also observed at positions S162N (addition of a glycosylation site, 6.25%) and A186T (receptor binding region, 6.25%). On the basis of amino acid substitutions in the HA1 domain, majority of the Turkish isolates were classified in the genetic group v and others in the genetic groups ii, iii, and vi. In the present study, we observed an increase in the variety and ratio of mutations detected in the HA1 and HA2 domains of the HA gene; however, these alterations have not yet resulted in vaccine escape mutants in Turkey. In addition, analysis of the NA regions of the isolates revealed that oseltamivir resistance was not an issue in Turkey.
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 66 (4), 299-305, 2013
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001206241142912
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- NII論文ID
- 130003381692
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 024716358
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- PubMed
- 23883840
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可