Detection of Five Rash-Associated Viruses Using Multiplex Real-Time PCR during 2006^|^ndash;2011

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  • Kaida Atsushi
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Kubo Hideyuki
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Sekiguchi Jun-ichiro
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Ohyama Minori
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Goto Kaoru
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Hase Atsushi
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences
  • Iritani Nobuhiro
    Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences

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  • Detection of Five Rash-Associated Viruses Using Multiplex Real-Time PCR during 2006–2011

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Many viruses have been reported to be associated with rash development. Multiplex real-time PCR was used to investigate the presence of 5 viruses associated with rashes: measles virus (MV), rubella virus (RV), human parvovirus B19 (PVB19), human herpes virus 6 (HHV-6), and HHV-7. A total of 187 clinical specimens from 169 patients with erythema were collected between January 2006 and December 2011. Virus-positive specimens were as follows: MV (n = 23), PVB19 (n = 8), RV (n = 2), HHV-6 (n = 5), HHV-7 (n = 1), MV and PVB19 (n = 1), and HHV-6 and HHV-7 (n = 1). All of the MV-positive specimens were collected in 2007 and the strains whose sequence were available (21/24, 87.5%) were of genotype D5. The results indicate that multiplex real-time PCR might be a useful screening method for detecting and differentiating rash-associated viruses in clinical specimens.

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