Separation of Dirofilaria immitis allergen from the IgG-inducing antigens

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  • SEPARATION OF <I>DIROFILARIA IMMITIS</I> ALLERGEN FROM THE IgG-INDUCING ANTIGENS<SUP>*</SUP>
  • Separation of Dirofilaria immitis aller

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Allergen in crude extract of Dirofilaria immitis was purified and separated from the IgG-inducing antigens by a combination of DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration.<BR>The molecular weight of the purified preparation was estimated to be approximately 20, 000 by gel filtration. The carbohydrate content of the preparation was apparently low, about 2%.<BR>Rats immunized with the allergenic fraction developed only a homocytotropic antibody and no hemagglutination antibody.

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