Development of a Diagnostic System for Novel Influenza A(H7N9) Virus Using a Real-Time RT-PCR Assay in Japan
-
- Takayama Ikuyo
- Influenza Virus Research Center, National Institute of Infectious Diseases
-
- Takahashi Hitoshi
- Influenza Virus Research Center, National Institute of Infectious Diseases
-
- Nakauchi Mina
- Influenza Virus Research Center, National Institute of Infectious Diseases
-
- Nagata Shiho
- Influenza Virus Research Center, National Institute of Infectious Diseases
-
- Tashiro Masato
- Influenza Virus Research Center, National Institute of Infectious Diseases
-
- Kageyama Tsutomu
- Influenza Virus Research Center, National Institute of Infectious Diseases
Search this article
Abstract
The first human cases of infection with avian influenza A(H7N9) virus were reported in March 2013 in China. The number of confirmed cases continues to increase, although almost all the cases are limited to China. In this study, a one-step real-time RT-PCR assay was developed for detecting the novel A(H7N9) virus. This assay was shown to have high specificity, good linearity, and high sensitivity to a broad range of Eurasian H7 viruses. The assay is useful both for diagnostic purposes in cases of suspected human infection with the influenza A(H7N9) virus and in the surveillance of both avian and human influenza viruses. A diagnostic system using this assay was prepared at 74 prefectural and municipal public health institutes and 16 quarantine stations in Japan early into the human H7N9 infection outbreaks, enabling potential diagnoses of H7N9 infection across Japan.
Journal
-
- Japanese Journal of Infectious Diseases
-
Japanese Journal of Infectious Diseases 68 (2), 113-118, 2015
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206241844864
-
- NII Article ID
- 40020410956
- 130004716745
-
- NII Book ID
- AA1132885X
-
- ISSN
- 18842836
- 13446304
-
- NDL BIB ID
- 026280978
-
- PubMed
- 25420657
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- Abstract License Flag
- Disallowed