First Detection of an <i>Escherichia coli</i> Strain Harboring the <i>mcr-1</i> Gene in Retail Domestic Chicken Meat in Japan

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  • Ohsaki Yusuke
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Hayashi Wataru
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Saito Satomi
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Osaka Shunsuke
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Taniguchi Yui
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Koide Shota
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine
  • Kawamura Kumiko
    Department of Pathophysiological Laboratory Science, Nagoya University Graduate School of Medicine
  • Nagano Yukiko
    Department of Bacteriology, Nagoya University Graduate School of Medicine
  • Arakawa Yoshichika
    Department of Bacteriology, Nagoya University Graduate School of Medicine
  • Nagano Noriyuki
    Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine

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Other Title
  • First Detection of an Escherichia coli Strain Harboring the mcr-1 Gene in Retail Domestic Chicken Meat in Japan
  • First detection of Escherichia coli harboring mcr-1 gene from retail domestic chicken meat in Japan

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Abstract

<p>Global spread of the plasmid-mediated colistin resistance gene, mcr-1 poses a challenge to public health because colistin is the last-line-of-defense against severe infections of multidrug-resistant Gram-negative bacteria. In Japan, a few studies have reported the prevalence of mcr-1 among food animal-derived Escherichia coli isolates, but the prevalence of mcr-1 in retail meats is not well known. We report here the first detection of mcr-1 in retail chicken meat. A total of 70 extended-spectrum beta-lactamase-producing E. coli isolates, recovered from retail chicken meats between August 2015 and June 2016, were screened for mcr-1. We found 1 CTX-M-1 beta-lactamase-producing E. coli isolate belonging to ST1684, phylogroup A. The mcr-1 gene was not located on an IncI1 plasmid encoding the blaCTX-M-1 gene. However, whole plasmid sequencing revealed that mcr-1 was located on an IncI2 plasmid. The sequences of the nikB-mcr-1-pap2-ydfA-topB region of the IncI2 plasmid in this study was almost identical to that of the previously described IncI2 plasmid, pECJS-61–63 present in E. coli isolated from pig feces in China, except for containing a synonymous mutation in the mcr-1 gene. Plasmid carrying the mcr-1 gene have not yet been identified in human isolates in Japan. Thus, strict monitoring or surveillance of colistin resistance among Gram-negative bacteria recovered from retail meat of food animals under colistin pressure, and humans, is crucial.</p>

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 70 (5), 590-592, 2017

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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