DNA-based characterization of an invasive mealybug (Hemiptera: Pseudococcidae) species damaging cotton in Pakistan
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- Ashfaq Muhammad
- National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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- Noor Ali Raza
- National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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- Mansoor Shahid
- National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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Mealybug (Hemiptera: Pseudococcidae) has emerged as a new cotton pest in Pakistan in recent years. We used DNA nucleotide sequences and PCR-RFLP, respectively, as tools for mealybug characterization and species composition. Partial nucleotide sequences of nuclear (elongation factor-1 α, ribosomal DNA subunits 18S and 28S) and mitochondrial (COI) genes were used for species characterization, and the combined ITS1-5.8S-ITS2 sequences of rDNA were used for PCR-RFLP analyses. Homology searches of the nuclear genes indicated that the mealybug species damaging cotton in Pakistan belonged to the genus Phenacoccus. Further, the barcoding sequence of COI showed a significant nucleotide similarity with Phenacoccus solenopsis. PCR-RFLP analysis with three different endonucleases did not show restriction site differences among mealybug individuals collected from various host plants and geographical locations. This is the first DNA-based characterization of cotton mealybug from Pakistan and the findings will help in decision making while considering a biological control program.
収録刊行物
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- Ａｐｐｌｉｅｄ Ｅｎｔｏｍｏｌｏｇｙ ａｎｄ Ｚｏｏｌｏｇｙ
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Ａｐｐｌｉｅｄ Ｅｎｔｏｍｏｌｏｇｙ ａｎｄ Ｚｏｏｌｏｇｙ 45 (3), 395-404, 2010
日本応用動物昆虫学会
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詳細情報 詳細情報について
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- CRID
- 1390001206242294272
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- NII論文ID
- 130000327180
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- NII書誌ID
- AA00543238
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- ISSN
- 1347605X
- 00036862
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- NDL書誌ID
- 10793883
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可