Growth of Protein Crystal at Interface between Two Liquids Using Slow Cooling Method.
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- Adachi Hiroaki
- Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Watanabe Takahiro
- Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Yoshimura Masashi
- Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Mori Yusuke
- Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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- Sasaki Takatomo
- Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
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抄録
A new method has been developed to grow high-quality and large protein crystals. By a slow cooling method, tetragonal lysozyme crystals were grown from spontaneous nucleation at the interface of two liquids wherein the crystals had no contact with a growth vessel. The long and narrow shape of the crystals indicated that they had grown at a lower supersaturation, leading to high crystallinity. This method also made it possible to remove the crystals easily without causing any mechanical damage. A large lysozyme crystal 2.5 mm in length was obtained in 25 days from a seed crystal kept at the interface. The combination of growth at the interface between two liquids and controlled slow cooling is effective for the growth of high-quality and large protein crystals.
収録刊行物
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- Japanese Journal of Applied Physics
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Japanese Journal of Applied Physics 41 (6B), L726-L728, 2002
The Japan Society of Applied Physics
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詳細情報 詳細情報について
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- CRID
- 1390001206255396096
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- NII論文ID
- 110006349713
- 130004529782
- 210000052881
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- NII書誌ID
- AA10650595
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- ISSN
- 13474065
- 00214922
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- NDL書誌ID
- 6172756
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
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