EVALUATION OF POTENTIAL NEPHROTOXICITY OF ANTIBIOTICS. II

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  • UMEDA TAKASHI
    Department of Urology, Faculty of Medicine, The University of Tokyo
  • KOISO KENKICHI
    Department of Urology, Faculty of Medicine, The University of Tokyo

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Other Title
  • 抗生物質の腎毒性に関する研究 II
  • コウセイ ブッシツ ノ ジン ドクセイ ニカンスルケンキュウ 2 ヒト ジン
  • Effects on Protein Synthesis of Human Kidney
  • ヒト腎の蛋白代謝に及ぼす影響について

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Abstract

In order to assess the potential nephrotoxicity by various antibiotics with therapeutic concentration, effects of antibiotics administered to in vitro system were investigated in terms of protein synthesis and nucleic acid metabolism.<BR>Kanamycin, streptomycin, gentamicin and colistin added to the incubation medium at the concentration of 10 μg/ml with kidney slices were demonstrated to inhibit the incorporation of orotic acid-6-14C into nuclear RNA.<BR>The incorporation of orotic acid-6-14C into RNA of free polysomes prepared from human kidney slices was inhibited by kanamycin, streptomycin, gentamicin, colistin and chloramphenicol.<BR>Significant reduction of the incorporation of L-leucine-14C into free polysome fraction was noticed by kanamycin, streptomycin and gentamicin added directly to cell free protein-synthesis system.<BR>The incorporation of orotic acid-6-14C into RNA of bound polysome fraction prepared from human kidney slices was inhibited by kanamycin, streptomycin, gentamicin and colistin.<BR>Inhibition of the incorporation of L-leucine-14C into bound polysome fraction was noticed when kanamycin, gentamicin and colistin were added directly in cell-free system.

Journal

  • CHEMOTHERAPY

    CHEMOTHERAPY 25 (10), 2984-2987, 1977

    Japanese Society of Chemotherapy

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