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- 諸角 美由紀
- 北里大学大学院感染制御科学府感染制御科学
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- 岩田 敏
- 国立病院東京医療センター小児科
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- 遠藤 廣子
- 東北労災病院小児科
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- 大石 智洋
- 上越総合病院小児科
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- 大成 滋
- なかふかわ小児科
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- 川村 尚久
- 大阪労災病院小児科 大阪医科大学小児科
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- 黒木 春郎
- 医療法人永津会斎藤病院小児科
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- 小林 正明
- 小林小児科医院
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- 斎藤 洪太
- 斎藤小児科医院
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- 酒井 律子
- 酒井医院
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- 砂川 慶介
- 北里大学医学部感染症学講座
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- 田島 剛
- 博慈会記念総合病院小児科
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- 新田 雅彦
- 清恵会病院小児科 大阪医科大学小児科
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- 野々山 勝人
- 北里大学医学部感染症学講座
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- 小林 玲子
- 明治製菓株式会社薬品総合研究所
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- 千葉 菜穂子
- 北里大学大学院感染制御科学府感染制御科学
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- 生方 公子
- 北里大学北里生命科学研究所
書誌事項
- タイトル別名
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- New PCR for rapid identification of Mycoplasma pneumoniae in clinical specimens from children with respiratory tract infections
- specimens from children with respiratory tract infections
- 小児呼吸器感染症検体を用いて
この論文をさがす
説明
We designed a set of primers on the 16 S rRNA gene to directly detect Mycoplasma pneumoniae by PCR in clinical specimens from patients with respiratory infections. The sensitivity of the primers was 2 CFU/tube under the following conditions: 35 cycles of 15 sec at 94°C, 15 sec at 53°C and 15 sec at 72°C /cycle. We therefore judged the PCR results to be positive if the CFU for M. pneumoniae in a clinical specimen was 1.1×103. The results of the PCR were obtained in about 2.5 h. We then used the PCR to examine 783 clinical specimens (epipharynx [n=612], throat [n=141], ear discharge [n=12] etc.) collected from pediatric patients between May 2002 and January 2003. The PCR results were positive in 79 cases (10.1%); 58/291 cases (19.9%) corresponded to specimens from patients with pneumonia, 13/207 cases (6.3%) to specimens from patients with acute bronchitis, 2/130 cases (1.5%) to those from patients with acute pharyngitis and 2/45 cases (4.4%) to those from patients with acute upper respiratory infections. The percentage of PCRpositive cases was significantly higher among the patients with lower part respiratory tract infections (x2=53.3008, p=0.0000). Among the patients with pneumonia, 65 were diagnosed with M. pneumoniae based on a significant rise or high antibody titer for M. pneumoniae as determined by the PA or CF test, and 46 of these cases were PCR-positive (70.8%). We also investigated the correlation between the antibiotics used before PCR and the PCR results in these 65 cases. Of the total of 43 cases, 21 not treated with antibiotics before PCR and 22 cases given oral β-lactam agents ineffective against M. pneumoniae, 38 (88.4%) were PCR-positive, whereas only 3 (23.1%) of the specimens from 13 patients treated with macrolides or new quinolones effective against M. pneumoniae were PCR-positive. We concluded from these results, that the PCR is very useful in selecting suitable chemotherapeutic agents for patients not treated with antibiotics, however it may be necessary to consider the influence of days after to develop infection on PCR results.
収録刊行物
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- 日本化学療法学会雑誌
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日本化学療法学会雑誌 51 (5), 289-299, 2003
公益社団法人 日本化学療法学会
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詳細情報 詳細情報について
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- CRID
- 1390001206284928000
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- NII論文ID
- 130004298167
- 80015958816
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- NII書誌ID
- AN10472127
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- ISSN
- 18845886
- 13407007
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可