Continuous Production of β-D-Fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-D-glucopyranoside (N-Acetylsucrosamine) Using a Column Reactor Packed with β-Fructofuranosidase-containing Mycelia of Aspergillus oryzae Immobilized on a Porous Carrier

DOI Web Site Web Site 被引用文献8件 参考文献11件 オープンアクセス
  • Sato Hitoshi
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
  • Yokochi Saeko
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
  • Kasama Terumi
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
  • Hirano Takako
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
  • Hakamata Wataru
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University
  • Nishio Toshiyuki
    Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University Department of Chemistry and Life Science, College of Bioresource Sciences, Nihon University

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タイトル別名
  • Continuous Production of β-D-Fructofuranosyl-(2→1)-2-acetamido-2-deoxy-α-D-glucopyranoside (<i>N</i>-Acetylsucrosamine) Using a Column Reactor Packed with β-Fructofuranosidase-containing Mycelia of <i>Aspergillus oryzae</i> Immobilized on a Porous Carrier
  • Continuous Production of ^|^beta;-D-Fructofuranosyl-(2^|^rarr;1)-2-acetamido-2-deoxy-^|^alpha;-D-glucopyranoside (N-Acetylsucrosamine) Using a Column Reactor Packed with ^|^beta;-Fructofuranosidase-containing Mycelia of Aspergillus oryzae Immobilized on a Porous Carrier
  • Continuous production of β-D-fructofuranosyl-(2↔1)-2-acetamide-2-deoxy-α-D-glucopyranoside (N-acetylsucrosamine) using a column reactor packed with β-fructofuranosidase-containing mycelia of Aspergillus oryzae immobilized on a porous carrier

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説明

We used Aspergillus oryzae mycelia containing a transfructosylation-catalyzing enzyme, β-fructofuranosidase, to establish a methodology for the large-scale production of N-acetylsucrosamine in a column bioreactor. A. oryzae mycelia containing the enzyme were grown in liquid culture and immobilized on Perlite macro-porous amorphous volcanic glass beads, which were then packed into the column reactor. Passing a solution containing 10% sucrose and 20% 2-acetamido-2-deoxy-D-glucose through the reactor resulted in the steady production of N-acetylsucrosamine for at least 30 days and provided a yield of at least 27.5% (molar yield) from sucrose. N-Acetylsucrosamine was isolated easily and in high yield (from 83.4 to 96.9%) from the column reactor eluate after preprocessing by yeast β-fructofuranosidase or by whole yeast cells.

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