Strategic Approach in the Practical Use of Industrial Carbohydrate Active Enzymes

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Original enzymes screened from nature are not necessarily perfect for industrial applications. Thermostable enzymes from hyperthermophiles often show broad reaction/substrate specificities. Although the enzymes from plants or mesophilic microorganisms exhibit relatively strict reaction/substrate specificities, their thermostabilities are not high enough for industrial purposes. Here we describe two practical approaches for overcoming this problem, introducing amylose production with α-glucan phosphorylases and cycloamylose production with amylomaltases.

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