Colorimetric quantification of α-<sc>d</sc>-mannose 1-phosphate
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- Nihira Takanori
- Faculty of Agriculture, Niigata University
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- Suzuki Erika
- Faculty of Agriculture, Niigata University
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- Kitaoka Motomitsu
- National Food Research Institute, National Agriculture and Food Research Organization
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- Nishimoto Mamoru
- National Food Research Institute, National Agriculture and Food Research Organization
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- Ohtsubo Ken’ichi
- Faculty of Agriculture, Niigata University
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- Nakai Hiroyuki
- Faculty of Agriculture, Niigata University
書誌事項
- タイトル別名
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- Colorimetric Quantification of α-D-Mannose 1-Phosphate
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抄録
We developed an enzymatic colorimetric method for the quantification of α-D-mannose 1-phosphate by adding phosphomannomutase, mannose 6-phosphate isomerase and glucose 6-phosphate isomerase to a conventional glucose 6-phosphate assay using glucose 6-phosphate dehydrogenase. In this method, α-D-mannose 1-phosphate is converted into D-glucose 6-phosphate via D-mannose 6-phosphate and D-fructose 6-phosphate and the resultant D-glucose 6-phosphate is ultimately converted into 6-phosphogluconolactone under concomitant reduction of thio-NAD+ to thio-NADH, which can be quantified by its wavelength of 400 nm. This method is not altered by the presence of D-mannose, D-mannosamine, N-acetyl-D-mannosamine, L-mannose, β-1,4-mannobiose, α-1,2-mannobiose, methyl α-D-mannoside or dimethyl sulfoxide and it would be useful in studies involving enzymes such as phosphorylases belonging to glycoside hydrolase family 130, which release α-D-mannose 1-phosphate as the reaction product.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 60 (2), 137-139, 2013
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206291898624
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- NII論文ID
- 10031159346
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- NII書誌ID
- AA11809133
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- COI
- 1:CAS:528:DC%2BC3sXhsVersbrI
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 024517539
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可