２種類の窒素源飼料を摂取した普通および無菌マウスにおける代謝性糞窒素量の測定

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  • Measurement of Metabolic Fecal Nitrogen in Conventional and Germ-free Mice Given Diets with Two Types of Nitrogen Source.
  • 2シュルイ ノ チッソゲン シリョウ オ セッシュシタ フツウ オヨビ ムキン

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Protein nitrogen from undigestible food residue is not present in the feces of mice fed a diet containing an amino acid mixture (AA) as the nitrogen (N) source, and protein N from in testinal microbes and related materials is notpresent in the feces of germ-free (GF) mice. In order to study fecal proteins, a WE diet containing 6, 10% or 14% (as N (%) ×6.25) purified whole-egg protein (WE), AA diets with the same amino acid composition as the WE diet, and a protein-free diet were given to conventional (CV) and GF mice. Protein (as protein N (%) ×6.25) excretion in the feces was then compared between mice fed the WE and AA diets or between CV and GF mice. In general, protein excretion in the feces of the WE diet-fed mice was much higher than that of the AA diet-fed mice, and a larger quantity of protein was excreted into the feces of CV mice than into the feces of GF mice. Fecal protein excretion differed according to the N content of the diet; mice fed the 14% diet showed the highest level, whereas micefed the protein-free diet showed the lowest. The fecal protein originating from undigestible foodresidue and intestinal microbes was related to the dietary N content, and mice fed diets with a low N content excreted less protein in the feces. However, excretion of protein originating from debris of intestinal mucosa and digestive juice was not related to the dietary N content. The protein corresponding to metabolic fecal N in the feces, i. e., protein of feces less the protein originating undigestible food residue (as protein) in the feces of the CV mice fed the AA diet, per g offood dry matter intake was as follows: proteinfree diet, 7.7mg; 6% diet, 8.4mg; 10% diets, 9.6 and 11.7mg; 14% diet, 11.2mg. The protein corresponding to metabolic fecal N per 100g dry matter in the feces was as follows: proteinfree diet, 4.49g; 6% diet, 5.42g; 10% diets, 6.37 and 7.28g; 14% diet, 7.31g.

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