Studies on Cyclodextrin Glucanotransferase from Brevibacterium sp. No. 9605.
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- Mori Shigeharu
- Amano Pharmaceutical Co., Ltd.
Bibliographic Information
- Other Title
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- Byevibacterium sp.No.9605株の生産するサイクロデキストリングルカノトランスフェラーゼに関する研究
- Brevibacterium sp. No.9605株の生産するサイクロデキストリングルカノトランスフェラーゼに関する研究
- Brevibacterium sp. No.9605カブ ノ セイサン スル サイクロデキストリン グルカノトランスフェラーゼ ニ カンスル ケンキュウ
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Abstract
A strain of Brevibacterium sp. No. 9605 was isolated from soil and produced cyclodextrin glucanotransferase (CGTase, EC. 2.4.1.19). The CGTase produced mainly γ-cyclodextrin (γ-CD) from sarch in the initial stage of reaction, but then, the proportion of β-CD was increased and also produced a small amount of α-CD. The CDs formation was affected by the presence of calcium ion and ethanol . In the presence of ethanol, the yield of γ-CD from soluble starch was increased . It was thought to be due to the repression of β-CD production from starch and the repression of γ-CD degradation by coupling reaction. Brevibacterium CGTase showed a broad acceptor specificity to various monosaccha rides and phenolic compounds and produced more transfer products of D-mannose, L-rhamnose, 1, 3-dihydroxybenzene, 1, 3, 5-trihydroxybenzene, 3-hydroxybenzyl alcohol, and (+) -catechin using as acceptors than Bacillus macerans and Bacillus stearothermophilus CGTases did. The transfer products of D-mannose and kojic acid were 4-O-α-D-glucosyl-D-mannose and kojic acid 7-O-α-D-glucoside, respectively.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (1), 87-95, 1999
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206293386880
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- NII Article ID
- 10008258742
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- NII Book ID
- AN10453916
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- ISSN
- 18807291
- 13447882
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- NDL BIB ID
- 4785651
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed