Colloidal Particle Recovery Using Extended and Shrunken Dextran Generated from Immobilized Enzyme in Porous Membrane
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- Kawasaki Hironobu
- Department of Applied Chemistry, Saga University
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- Seto Hirokazu
- Department of Applied Chemistry, Saga University
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- Ohto Keisuke
- Department of Applied Chemistry, Saga University
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- Kawakita Hidetaka
- Department of Applied Chemistry, Saga University
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説明
A colloidal particle rejection and recovery system using extended and shrunken dextran was proposed. Dextransucrase forms a complex with dextran in an enzymatic reaction with sucrose; therefore dextran is easily formed on membrane pores. A dextransucrase solution was permeated through a Shirasu porous glass membrane at various permeation rates. A sucrose solution was permeated to generate dextran from the active sites of DSase immobilized on the membrane pores, at an immobilized density of 1.9-23 U/g, by controlling the dextran density; the immobilized density of DSase was high when immobilization was performed at a low permeation rate, resulting in a high generated-dextran density of 9.6-28 mg/g. A colloidal particle solution was permeated through the membrane for rejection and recovery. The high-density dextran rejected colloidal particles efficiently. In 50% methanol solution, the rejected colloidal particles leaked through the shrunken dextran structure to achieve smart recovery of colloidal particles.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 59 (1), 31-35, 2011
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206293402496
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- NII論文ID
- 10030138341
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- NII書誌ID
- AA11809133
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 023567019
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可