Purification and Characterization of Pectate Lyase I and II from Fungus Stereum purpureum.
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- Miyairi Kazuo
- Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University
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- Nishida Kaori
- Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University
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- Takarae Masaru
- Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University
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- Shikanai Yumiko
- Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University
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- Okuno Toshikatsu
- Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University
Bibliographic Information
- Other Title
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- 銀葉病菌のペクチン酸リアーゼI とII の精製と性質
- Purification and Characterization of Pectate Lyase 1 and 2 from Fungus Stereum purpureum
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Abstract
Two pectate lyases (PLs I and II) were isolated from the culture filtrate of Stereum purpureum, the causative fungus of apple silver-leaf disease. The lyases were isolated using DE52 column chromatography, and their molecular masses were estimated to be 48 kDa by SDS-PAGE. After treatment with endo-β-N-acetylglucosaminidase (Endo H), their molecular masses decreased equally to 38 kDa, indicating that these PLs are glycoproteins. The pI values for PLs I and II were determined to be 4.15 and 4.10, respectively. The amino acid compositions of PLs I and II were very similar, and furthermore the amino acid sequences of the N-terminal 20 residues of the two enzymes were identical. Their optimum pH values, and thermal and pH stabilities were 9.5, up to 50°C, and pH 6-7, respectively. Both PLs I and II required 0.2 mM Ca2+ ion for maximum activity. The enzymes were assigned as endo-types because of the detection of oligo-GalUAs as products from the initial reaction. However they mainly formed products less than octa-GaIUA in degree of polymerization. Erwinia carotovora PL mainly formed products more than octa-GalUA. Therefore they were different in their initial reaction products. As in the case of the E. carotovora PL, the final products of S. purpureum PL were 4, 5-unsaturated di- and tri-GalUA.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 49 (2), 99-106, 2002
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206293546368
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- NII Article ID
- 10008253442
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- NII Book ID
- AN10453916
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- COI
- 1:CAS:528:DC%2BD38Xkt1Cisrg%3D
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- ISSN
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
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- NDL BIB ID
- 6142235
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed