Colorimetric Quantification of β-(1→4)-Mannobiose and 4-<i>O</i>-β-D-Mannosyl-D-glucose
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- Jaito Nongluck
- Research Faculty of Agriculture, Hokkaido University
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- Saburi Wataru
- Research Faculty of Agriculture, Hokkaido University
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- Muto Hirohiko
- Research Faculty of Agriculture, Hokkaido University
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- Matsui Hirokazu
- Research Faculty of Agriculture, Hokkaido University
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- Mori Haruhide
- Research Faculty of Agriculture, Hokkaido University
Bibliographic Information
- Other Title
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- Colorimetric Quantification of β-(1→4)-Mannobiose and 4-O-β-D-Mannosyl-D-glucose
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Abstract
Spectrophotometric quantification method of carbohydrates is useful for processing multiple samples. In this study, we established colorimetric quantification for 4-O-β-D-mannosyl-D-glucose (Man-Glc) and β-(1→4)-mannobiose (Man2). For quantification of Man-Glc, phosphorolysis of Man-Glc catalyzed by 4-O-β-D-mannosyl-D-glucose phosphorylase (MGP) was coupled with quantification of D-glucose by the glucose oxidase-peroxidase method. In addition to MGP, cellobiose 2-epimerase (CE) was added for quantification of Man2. In both quantifications, a good linear relationship was obtained between A505 and the sample concentration (0-0.5 mM). The A505 values obtained at various concentrations of Man2 and Man-Glc were almost identical to those with equivalent D-glucose concentrations. Kinetic parameters of Ruminococcus albus and Rhodothermus marinus CEs for the epimerization of Man2 were determined using the quantification method for Man-Glc. Both enzymes showed 5-15-fold higher kcat/Km values than those for cellobiose and lactose, which supports the prediction that these enzymes utilize Man2 as a substrate in the β-mannan metabolic pathway.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 61 (4), 117-119, 2014
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206293617280
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- NII Article ID
- 130004699355
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- NII Book ID
- AA11809133
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- ISSN
- 18807291
- 13447882
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- HANDLE
- 2115/67698
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- NDL BIB ID
- 025924313
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed