Nucleotide Sequence of a Paenibacillus Endoglucanase Gene and Characterization of the Recombinant Enzyme
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- Ogawa Akinori
- Tochigi Research Laboratories of Kao Corporation
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- Sumitomo Nobuyuki
- Tochigi Research Laboratories of Kao Corporation
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- Hakamada Yoshihiro
- Department of Applied Bioscience, College of Bioscience and Chemistry, Kanazawa Institute of Technology
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- Saeki Katsuhisa
- Tochigi Research Laboratories of Kao Corporation
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- Ozaki Katsuya
- Tochigi Research Laboratories of Kao Corporation
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- Kobayashi Tohru
- Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Bibliographic Information
- Other Title
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- <i>Paenibacillus</i> 属細菌由来のエンドグルカナーゼ遺伝子のクローニングと組換え酵素の性質
Abstract
An endoglucanase gene of Paenibacillus sp. strain KSM-N546 was cloned and sequenced. The gene for the endoglucanase (Egl-546H) had an open reading frame of 1710 bp that encoded a putative signal sequence (37 amino acids) and mature enzyme (533 amino acids: 61,348 Da). The mature enzyme was most homologous to a cellulase of Geobacillus sp. Y412MC10 with 68% identity. The optimal pH and temperature of the recombinant enzyme for degrading carboxymethyl cellulose (CMC) were around pH 5.3 and 40°C, respectively. The enzyme efficiently degraded CMC and lichenan as well as glucomannan. It was crystallized with a rod shape by a hanging drop vapor-diffusion method. Egl-546H is comprised of two conserved domains, a glycosyl hydrolase family 5 domain and a DUF291 domain of unknown function. Several mutant proteins deleted of a DUF291-like domain were produced by Escherichia coli as inclusion bodies with no enzymatic activity.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 56 (4), 253-259, 2009
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390001206293848960
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- NII Article ID
- 130004480943
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- COI
- 1:CAS:528:DC%2BD1MXhsVOks7zN
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- ISSN
- 18807291
- 13447882
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed