Thermoanaerobacter brockii由来トレハロースホスホリラーゼによるα-D-ガラクトシルα-D-グルコシドの効率的酵素合成
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- 茶圓 博人
- Hayashibara Biochemical Laboratories, Inc
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- 仲田 哲也
- Hayashibara Biochemical Laboratories, Inc
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- 向井 直子
- Hayashibara Biochemical Laboratories, Inc
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- 西本 友之
- Hayashibara Biochemical Laboratories, Inc
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- 福田 恵温
- Hayashibara Biochemical Laboratories, Inc
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- 杉本 利行
- Hayashibara Biochemical Laboratories, Inc
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- 栗本 雅司
- Hayashibara Biochemical Laboratories, Inc
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- 辻阪 好夫
- Hayashibara Biochemical Laboratories, Inc
書誌事項
- タイトル別名
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- Efficient Enzymatic Synthesis of Disaccharide, .ALPHA.-D-Galactosyl .ALPHA.-D-Glucoside, by Trehalose Phosphorylase from Thermoanaerobacter brockii.
- Efficient Enzymatic Synthesis of Disaccharide,α-D-Galactosyl α-D-Glucoside,by Trehalose Phosphorylase from Thermoanaerobacter brockii
- Efficient Enzymatic Synthesis of Disaccharide アルファ D Galactosyl アルファ D Glucoside by Trehalose Phosphorylase from Thermoanaerobacter brockii
この論文をさがす
抄録
A non-reducing disaccharide was synthesized by trehalose phosphorylase from a thermophilic anaerobe, Thermoanaerobacter brockii, using trehalose as a glucosyl donor and D-galactose as an acceptor.This disaccharide was efficiently isolated from the reaction mixture by glucose eliminationusing baker's yeast, trehalase digestion, alkaline treatment and preparative HPLC. The chemicalstructure of the disaccharide was determined to be α-D-galactopyranosyl α-D-glucopyranoside byacid hydrolysis, methylation analysis, and by 13C-NMR spectrometry. This saccharide was onlyminimally hydrolyzed by α-galactosidase, but not hydrolyzed by α- or β-glucosidase, β-galactosidase and trehalase.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 48 (2), 135-137, 2001
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206294116096
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- NII論文ID
- 10008252541
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- NII書誌ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
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- COI
- 1:CAS:528:DC%2BD3MXjslGnurY%3D
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- NDL書誌ID
- 5764418
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可