Expression of PTH/PTHrP Receptor mRNA in the Kidney of Rats Fed High Phosphorus Diet.

KAJITA Yasutaka, MASUYAMA Ritsuko, UEHARA Mariko, SUZUKI Kazuharu, GOTO Shiro

doi:10.4327/jsnfs.50.217

Parathyroid hormone (PTH) is known to regulate calcium and phosphorus metabolism. PTH activates the PTH/PTH-related peptide (PTHrP) receptor which is widely distributed in kidney, bone and various other tissues, and increases the activity of adenylate cyclase and phospholipase C. Our previous study suggested a decreased response of PTH in the kidney of rats fed a high phosphorus diet (P level 1.5%) compared with that in rats fed a control diet (P level 0.5%). In the present study, expression of PTH/PTHrP receptor mRNA was investigated in rat kidney, since alteration of the PTH/PTHrP receptor is one of the reasons for down-regulation of PTH action. A high phosphorus diet induced an increased of PTH, but the urinary excretion of cyclic 3′5′-adenosine monophosphate (cAMP) /creatinine was significantly decreased and the serum 1α, 25 (OH) ₂D₃ level tended to decrease. PTH/PTHrP receptor mRNA expression in the kidneys of rats fed the high-phosphorus diet decreased significantly on the 21st day. These data suggest that the down-regulation of PTH is caused by a decrease in PTH/PTHrP receptor mRNA expression, and that PTH/PTHrP receptors are decreased.

Expression of PTH/PTHrP Receptor mRNA in the Kidney of Rats Fed High Phosphorus Diet.

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