Purification and some Properties of Human Urinary Follicle Stimulating Hormone
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- OKUMURA HAJIME
- Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.
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- NAMBA SHUICHI
- Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.
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- MATSUSHIMA SANAE
- Research Laboratories, Teikoku Hormone Mfg. Co., Ltd.
Bibliographic Information
- Other Title
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- Purification and some properties of human urinary follicle stimulating hormon
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Abstract
A new approach to the preparation of biologically pure human urinary follicle stimulating hormone from human menopausal urine was performed. A crude human menopausal gonadotropin (HMG) with an activity of about 80-90 IU per mg was applied to a column filled with DEAE-cellulose, which had been previously equilibrated with 0.005M ammonium acetate buffer, pH 8.5. By a sodium chloride gradient' method on a variable gradient apparatus, the fraction of 0.08-0.12M sodium chloride concentration had 3 to 4 times higher in specific activity than the starting material. The active fraction was pooled, and was further purified by the gel filtration on Sephadex G-75 and the chromatography on CM-Sephadex C-25. This procedure yielded urinary FSH with an activity of more than 830 IU per mg.<BR>A homogeneous ultracentrifuge pattern was observed with this purified product, however, only faint-stained minor components still remained in the disc gel electrophoresis for further investigation. An isoelectric point of this. product estimated by the electrofocusing method was about pH 4.0. The LH/FSH ratio of our purified urinary FSH was less than 0.1.
Journal
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- Endocrinologia Japonica
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Endocrinologia Japonica 20 (2), 97-101, 1973
The Japan Endocrine Society
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Keywords
Details 詳細情報について
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- CRID
- 1390001206297557376
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- NII Article ID
- 130000956644
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- NII Book ID
- AA00635052
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- ISSN
- 21856370
- 00137219
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- NDL BIB ID
- 7672864
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- PubMed
- 4740603
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed