MODE OF OCCURRENCE OF STEROID HORMONES IN THE BLOOD SERUM (II)

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  • OKADA HISASHI
    Endocrinological Laboratories, School of Medicine, Gunma University

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  • MODE OF OCCURRENCE OF STEROID HORMONES IN THE BLOOD SERUM

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1. present study disclosed that relatively small amount of steroids are contained in the fraction of Cohn IV-1.<BR>2. Steroids examined were of conjugated form with α-globulin or β-globulin, not being in free forms.<BR>3. Such bound steroids were dissociated from the serum protein in the treatment with P. E. and isolated steroid moved to parts of IVp and Vp, which were characteristic of m-dinitrobenzene treatment, which disappeared in BPB staining, determined by Coleman's nepho-colorimeter after color development with m-DNB.<BR>4. IVp and Vp represented the curves originated in androgenic steroid hormones and corticoid hormones respectively.

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