Biochemical studies on human salivary proteins.-2-Preliminary characterization of a fraction with hypocalcemic and leukocytosis-promoting activities in the digest of salivaparotin-A by pronase P

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  • KUBOTA YUKIHO
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
  • UEKI HIROSHI
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
  • ITO YOSOJI
    Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo

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  • BIOCHEMICAL STUDIES ON HUMAN SALIVARY PROTEINS II. PRELIMINARY CHARACTERIZATION OF A FRACTION WITH HYPOCALCEMIC AND LEUKOCYTOSIS-PROMOTING ACTIVITIES IN THE DIGEST OF SALIVA-PAROTIN-A BY PRONASE P

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Fractionation of the digest of saliva-parotin-A by Pronase P was carried out by gel filtration on Sephadex G-25 column to give five fractions (P-1--V), using 1M acetic acid as eluant. Of five fractions, only P-I containing peptides of fairly large molecular weights showed potent hypocalcemic and leukocytosis-promoting activities in rabbits. Electrophoretical behavior and amino acid pattern of P-I were quite different from those of saliva-parotin-A.

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