- 【Updated on May 12, 2025】 Integration of CiNii Dissertations and CiNii Books into CiNii Research
- Trial version of CiNii Research Knowledge Graph Search feature is available on CiNii Labs
- 【Updated on June 30, 2025】Suspension and deletion of data provided by Nikkei BP
- Regarding the recording of “Research Data” and “Evidence Data”
Methods of electrophoretical application of estrogen to a single neuron of the brain under in vivo and in vitro conditions.
-
- YAMADA YASUYUKI
- Electrophysiological Laboratory of Research Center, School of Medicine, Kanazawa University
-
- UESHIMA HANJI
- Department of Obstetrics and Gynecology, Ishikawa Saiseikai Hospital
Bibliographic Information
- Other Title
-
- Methods of Electrophoretical Application of Estrogen to a Single Neuron of the Brain under in vivo and in vitro Conditions(Note)
- Methods of Electrophoretical Applicatio
Search this article
Description
The techniques of the electrophoretical study of hormone sensitivities of neurons were examined in vivo and in vitro. For the in vivo study, estrogen and other hormones were applied electrophoretically to neurons of the rat brain of both sexes under urethane anesthesia. Some neurons were sensitive to estrogen and their activities were all inhibited. These neurons were distributed in the hypothalamus and the septal nucleus of the limbic system. So the in vivo method affords direct evidence for the presence of estrogen-sensitive neurons in the brain. Then a useful method was found to study the similar effects under the microscopic observation of a single neuron instead of stereotaxic insertion of the microelectrode. By this in vitro method the chemicals and hormones are able to be applied to any point on a single neuron through the micropipette and the medium composition around the neuron can be variously changed at the same time.
Journal
-
- Endocrinologia Japonica
-
Endocrinologia Japonica 25 (4), 397-401, 1978
The Japan Endocrine Society
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206299677440
-
- NII Article ID
- 130000954766
-
- NII Book ID
- AA00635052
-
- ISSN
- 21856370
- 00137219
-
- NDL BIB ID
- 1955970
-
- PubMed
- 710374
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- JaLC
- NDL Search
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
-
- Abstract License Flag
- Disallowed