Effects of parathyroid hormone and prostaglandin E1 in vitro on release of cyclic AMP from kidney cortical tissue.

DOI Web Site Web Site PubMed
  • ARAKI NOBUKO
    Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology
  • NAGATA NAOKAZU
    Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology
  • KIMURA NARIMICHI
    Clinical Division, Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology

書誌事項

タイトル別名
  • Effects of Parathyroid Hormone and Pros

この論文をさがす

抄録

The interaction of parathyroid hormone-(PTH) and prostaglandin E1 (PGE1) on cyclic AMP metabolism in the rat renal cortical tissue was examined in vitro, placing an emphasis on the process of release of the nucleotide from the tissue. PTH enhanced the release of cyclic AMP from cells even in the presence of 10μM cyclic AMP added to the incubation medium. This effect of PTH to accelerate cyclic AMP release was suppressed by simultaneous addition of PGE1. Although PTH and PGE1 showed an additive effect in stimulating the adenylate cyclase activity of the purified renal plasma membrane, in the experiment in which slices were employed the sum of cyclic AMP in the tissue and medium under simultaneous stimulation tended to be less than that under the stimulation by PTH alone. However, the increase by PTH of specific activity of cyclic AMP produced in slices prelabeled with [14C] adenine was further enhanced by the simultaneous addition of PGE1, suggesting that PGE1, by suppressing the release of cyclic AMP, keeps more cyclic AMP intracellularly and offers phosphodiesterase more substrate. It seemed that the release of cyclic AMP from the kidney cortical tissue could proceed against a concentration gradient and was regulated somehow by PTH and PGE1. It was noted also that the change in the release process influenced the intracellular metabolism of cyclic AMP, indicating the importance of taking such an extracellular release mechanism into consideration in the study on the cellular metabolism of cyclic AMP.

収録刊行物

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ