SSR およびゲノムスキャニング (RLGS) 法を用いたモモ枝変わり品種の解析

  • 間瀬 誠子
    独立行政法人農業・食品産業技術総合研究機構果樹研究所
  • 池谷 祐幸
    独立行政法人農業・食品産業技術総合研究機構果樹研究所
  • 佐藤 義彦
    独立行政法人農業・食品産業技術総合研究機構果樹研究所

書誌事項

タイトル別名
  • Analysis of Bud Sport Cultivars of Peach (Prunus persica (L.) Batsch) by Simple Sequence Repeats (SSR) and Restriction Landmark Genomic Scanning (RLGS)

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説明

To detect genetic mutations in bud sport cultivars of peach, RLGS (restriction landmark genomic scanning) analysis was performed on 7 putative bud sport mutant cultivars after SSR (simple sequence repeats) analysis for identifying false-mutants. Three cultivars, ‘Yawata Hakuho’, ‘Odoroki’, and ‘Kanoiwa Hakuto’, postulated to be bud sport mutants, were confirmed as false-mutant cultivars. This suggests that other false-mutant strains of peach are also likely to be registered with the Plant Variety Authority as bud mutant cultivars. A stable RLGS profile of peach, obtained using NotI as a landmark enzyme, showed about 400 spots in a single experiment. Comparing the profiles between mutant and original cultivars, polymorphic spots were discovered in 2 mutatnt cultivars, ‘Gyosei’ and ‘Nagasawa Hakuho’, but were not detected by SSR analysis. RLGS was thus considered to be an effective method to detect very small genomic variations in mutant strains.<br>

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 76 (1), 20-27, 2007

    一般社団法人 園芸学会

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