Induction of Plantlets from Anthers of‘Trovita’Orange

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  • ‘トロビタ’オレンジ葯からの植物体の分化
  • "トロビタ"オレンジ葯からの植物体の分化〔英文〕
  • トロビタ オレンジ ヤク カラ ノ ショクブツタイ ノ ブンカ エイブン
  • Induction of Plantlets from Anthers of^|^lsquo;Trovita^|^rsquo;Orange
  • <i>Citrus sinensis</i> Osbeck

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Abstract

Anther culture of‘Trovita’orange, a cultivar of sweet orange (Citrus sinensis Osbeck), was attempted.<br>Anthers with microspores at uninucleate stage were cultured on the Murashige and Skoog′s medium(9) supplemented with or without indole-3-acetic acid (IAA) and kinetin at 28°C in darkness. Embryoids appeared directly from within anthers after about 10 weeks of culture. The media containing kinetin and IAA both at 0.002 or 0.02mg/l were effective in embryoid formation. In addition, 0.2 or 2.0mg/l kinetin and 0.2mg/l IAA seemed to be also favorable when combined with 0.002 or 0.02mg/l IAA and 0.002 or 0.02mg/l kinetin, respectively. Calli were formed well on the media with kinetin at 2.0mg/l followed by those with IAA at 2.0mg/l.<br>After incubation in light with a 16-hour photoperiod, some embryoids differentiated shoots. Induction of roots was stimulated by transfer of embryoids from the embryoid induction media containing growth regulators to a medium without them.<br>Some root tip cells of differentiated plantlets had a diploid chromosome number of 18.

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