Transformation of Kiwifruit (Actinidia chinensis) and Trifoliate Orange (Poncirus trifoliata) with a Synthetic Gene Encoding the Human Epidermal Growth Factor (hEGF).

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  • Kobayashi Shozo
    Akitsu Branch, Fruit Tree Research Station
  • Nakamura Yuri
    Akitsu Branch, Fruit Tree Research Station
  • Kaneyoshi Junko
    Institute of Biotechnology, Hiroshima Prefectural Agricultural Research Center
  • Higo Hiromi
    Department of Molecular Biology, National Institute of Agrobiological Resources
  • Higo Ken-ichi
    Department of Molecular Biology, National Institute of Agrobiological Resources

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  • ヒト上皮細胞成長因子をコードした合成遺伝子を用いたキウイフルーツおよびカラタチの形質転換
  • Transformation of kiwifruit Actinidia c

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Abstract

Kiwifruit (Actinidia chinensis) and trifoliate orange (Poncirus trifoliata) were trans- formed with a chemically synthesized gene encoding the human epidermal growth factor (hEGF) under the control of the cauliflower mosaic virus 35S RNA gene promoter. The introduced gene (s) were expressed in the young leaves of the regenerated plants. The highest hEGF peptide content so far detected, by a sandwich enzyme-linked immunosor- bent assay for hEGF, was about 65 and 113 pg•mg-1 soluble proteins in kiwifruit and trifoliate orange leaves, respectively.<BR>This is the first demonstration of the production of human bioactive peptides, non- fused with other proteins, in fruit trees and is suggesting the possibility of producing some useful components in fruits by using transformation technique.

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