パパイア未受精胚珠からの不定胚誘導と植物体再生
書誌事項
- タイトル別名
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- Adventitious Embryogenesis and Plantlet Regeneration from Ovules of Unpollinated Ovaries of Papaya(Carica papaya L.).
- パパイア未受精はい珠からの不定はい誘導と植物体再生
- パパイア ミジュセイ ハイシュ カラ ノ フテイハイ ユウドウ ト ショクブツタイ サイセイ
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説明
Ovules were excised from unpollinated papaya ovaries before flowering and imbedded on the culture media containing cytokinin, auxin, and gibberellins. Adventitious embryos were induced only in the culture media with mixed gibberellins and GA3 in the dark. No embryo was initiated with cytokinin and auxin, whereas the gibberellin mixture (GA3>80%, GA1+GA4≒10%, minute amount of GA2+GA7), GA3, and GA4 individually promoted induction, but GA1, GA5, and GA7 were ineffective. The adventitious embryos were directly induced from the ovules. However, there were many abnormal embryos with malformed cotyledons. The induced embryos were transferred onto a hormone-free MS medium to regenerate plantlets. Plantlets, derived from embryos with gibberellin mixture, GA3, and GA4, and their respective percentages were 18.6, 26.4, and 26.9. The total number of surviving plants after acclimatization was only 44 (7.2%). All acclimated plants were diploid (2n=18), whereas some of the adventitious embryos were heteroploid and tetraploid (4n=36). RAPD analyses of the regenerated plants using primer RA-1 and RA-47 showed that several had differing banding patterns from those of their parents. Therefore, it is suggested that some of the regenerated plants were induced from haploidic reproductive cells.
収録刊行物
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- 園芸学会雑誌
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園芸学会雑誌 69 (2), 195-201, 2000
一般社団法人 園芸学会
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詳細情報 詳細情報について
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- CRID
- 1390001206301452160
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- NII論文ID
- 110001816379
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- NII書誌ID
- AN00025257
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- COI
- 1:CAS:528:DC%2BD3cXitFWjs7o%3D
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- ISSN
- 1880358X
- 00137626
- http://id.crossref.org/issn/00137626
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- NDL書誌ID
- 5312319
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- IRDB
- NDLサーチ
- Crossref
- NDLデジコレ（旧NII-ELS）
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
