ウメ (Prunus mume) の自家和合性分子マーカー S<SUB>f</SUB>-RNase の特性調査と cDNA クローニング

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献9件 参考文献15件

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  • Characterization and cDNA Cloning for Sf-RNase, a Molecular Marker for Self-compatibility, in Japanese Apricot(Prunus mume).

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Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a common S-RNase (Sf-RNase) gene that can be used as a molecular marker for self-compatibility. In this study, we further characterized Sf-RNase by comparing S-RNase of self-compatible 'Kensaki' (SfSf) and self-incompatible 'Nankou' (S1S7). cDNA libraries from the styles with stigmas of these two cultivars were constructed and cDNAs encoding Sf-, S1-, and S7-RNases were cloned. Deduced amino acid sequences from these cDNAs all contained two active domains of the T2/S type RNase family and five conserved regions of the rosaceous S-RNase. RNA blot analysis showed that the Sf-, S1-, and S7-RNase genes were transcribed in the pistil but not in the leaf as with other S-RNase genes of Prunus. Furthermore, 2D-PAGE analysis revealed that Sf-RNase has molecular mass, isoelectric point, and immunological characteristics similar to other S-RNase. These results indicate that the pollen-S gene that is supposedly tightly linked to the Sf-RNase gene may be responsible for the self-compatibility observed in the Sf-haplotype.

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  • 園芸学会雑誌

    園芸学会雑誌 71 (5), 595-600, 2002

    一般社団法人　園芸学会

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