Tracer Experiment Administering L-Phenylalaine-U-14C and L-Tyrosine-U-14C to the Tissue Slices of Bamboo Shoots

KOZUKUE Etsuko, MIZUNO Susumu

doi:10.2503/jjshs.56.222

Bibliographic Information

Other Title

タケノコ組織中へのL-フェニルアラニン-U-14C及びL-チロシン-U-14Cのトレーサー実験
タケノコ組織中へのL-フェニルアラニンーU-14C及びL-チロシンーU-14Cのトレーサー実験〔英文〕
タケノコソシキチュウヘノ L フェニルアラニン U 14C オヨビ L
Tracer Experiment Administering L-Phenylalaine-U-14C and L-Tyrosine-U-14C to the Tissue Slices of Bamboo Shoots

Search this article

Description

Uniformly ¹⁴C-labeled L-phenylalanine and L-tyrosine were administered to tissue slices of both top and base sections of bamboo shoots. Alcohol soluble substances were extracted and then separated into organic acid, sugar and amino acid fractions by ion exchange chromatography. The homogentisic acid fraction among the organic acids was collected by high-performance liquid chromatography (HPLC) and its radioactivity was measured, while the alcohol insoluble residue was used for the analysis of lignin aldehyde by the method of alkaline nitrobenzene oxidation. 1. The two labeled amino acids were steadily incorporated into the tissues during incubation and rapidly converted to organic acid, sugar and alcohol insoluble residue, especially the latter. 2. On determining the amount of phenylalanine converted to tyrosine, it was found that this was extremely small. 3. The incorporation of phenylalanine-U-¹⁴C into alcohol insoluble residue was higher than that of tyrosine in both sections. 4. Although the conversion into lignin aldehyde from phenylalanine-U-¹⁴C was higher than that from tyrosine-U-¹⁴C, it was found that tyrosine incorporated into the shoots was converted to a remarkable extent for formation of lignin aldehyde. 5. The incorporation of phenylalanine and tyrosine into homogentisic acid was very low. From these results, we assume that the conversion of phenylalanine to tyrosine or of tyrosine to homogentisic acid is very small, and that a part of the high amount of tyrosine in the shoots may be used for formation of lignin.

Journal

Journal of the Japanese Society for Horticultural Science

Journal of the Japanese Society for Horticultural Science 56 (2), 222-228, 1987

THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE

Keywords

Details 詳細情報について

Export

Report a problem

Tracer Experiment Administering L-Phenylalaine-U-<sup>14</sup>C and L-Tyrosine-U-<sup>14</sup>C to the Tissue Slices of Bamboo Shoots