カキ属数種の倍数性とゲノムサイズ

DOI NDLデジタルコレクション 日本農学文献記事索引 Web Site Web Site 被引用文献13件 参考文献22件

書誌事項

タイトル別名
  • Ploidy Level and Genome Size of Several Diospyros Species.
  • Ploidy level and genome size of several

この論文をさがす

抄録

Callus cells induced from leaf primordia were used for a rapid and efficient determination of genome size and ploidy level of several Diospyros spp. including D. kaki. Nuclear DNA contents of nine Japanese persimmon (Diospyros kaki Thunb.) cultivars and other 12 Diospyros species were estimated by flow cytometry. Compared to the known nuclear DNA contents of chicken red blood cells and tobacco leaf cells, genome sizes of hexaploid cultivars (2n=6x, x=15) of D. kaki were estimated to be 5.00-5.24 pg/2C and those of nonaploid (2n=9x) to be 7.51-8.12 pg/2C. Nuclear DNA content of another hexaploid species, D. virginiana, was 5.12 pg/2C and that of a tetraploid, D. rhombifolia, was 3.76 pg/2C. Nuclear DNA contents of all the diploid species tested, except for D. montana, fell in the range of 1.57-2.31 pg/2C. Although D. montana is a diploid, its genome size was 3.48 pg/2C and about the same size as that of a tetraploid, D. rhombifolia. There was a strong positive linear correlation between the ploidy levels and the genome sizes of Diospyros spp. tested, except for D. montana. In this study, chromosome number was also counted, using callus cells to determine if ploidy levels can be deduced from flow cytometry. D. kaki cv. Miyazakitanenashi was nonaploid with chromosome number of 2n=135, D. kaki cv. Jiro and D. virginiana were hexaploid with 2n=90, and D. rhombifolia was tetraploid with 2n=60, and the other species tested, including four species whose ploidy levels had been unknown, were determined to be diploid. The results agreed with the deduced ploidy levels from flow cytometry except for D. montana.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 67 (3), 306-312, 1998

    一般社団法人　園芸学会

被引用文献 (13)*注記

もっと見る

参考文献 (22)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ