Induced Polyploid Grapes via in vitro Chromosome Doubling.

DOI NDL Digital Collections JASI Web Site Web Site 8 Citations 32 References

Bibliographic Information

Other Title
  • 試験管内染色体倍加による人為倍数体ブドウの作出

Search this article

Abstract

In vitro chromosome doubling of the 29 diploid-, 3 triploid-, and 1 tetraploid-grape accession, Vitis spp. were performed using the axillary buds of growing shoots. Treatment with 0.05% colchicine for one or two days is suitable to induce tetraploids (4X). Shoots, arising from treated-buds, often exhibited abnormal leaves, and part of them turned out to be cytochimeras (2X+4X). Additional tetraploids were isolated from parts of cytochimeras by three-divided stem cuttings. A different response to colchicine was educed in diploid cultivars, and the percentage acquisition of induced tetraploids was relatively higher in V. vinifera than in the American hybrid. Induced hexaploids (6X) were found to have a less satisfactory root system, but cytochimera, having only 3X cells in root tips, rooted well. No induced octoploids (8X) were obtained, and the growth of cytochimera, partly having 8X cells, were slow and dwarfed. Induced tetraploids were vigorous, rooted easily, and stable over many years, whereas cytochimera of 3X+6X and 4X+8X were likely to be lost during ex vitro cultivation, reverting to the source triploid and tetraploid, respectively. A comparision of induced tetraploids and the source diploids revealed no significant difference in time of full maturity, cluster and berry shape, skin color, Brix, and acidity. However, depending on the variety, the increase in berry size in the 4X to 2X ranged from 1.1 to 1.5 fold with an average of 1.3.

Journal

Citations (8)*help

See more

References(32)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top